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. 2009 Jun;37(6):475-9.
doi: 10.1016/j.jdent.2009.03.004. Epub 2009 Apr 5.

Root dentin body moisture changes caused by ascorbic acid

Takashi Komabayashi  1 , Chul Ahn, Song Zhang, Qiang Zhu, Larz S W Spångberg
Affiliations

Root dentin body moisture changes caused by ascorbic acid

Takashi Komabayashi et al. J Dent. 2009 Jun.

Abstract

Objectives: To quantify in vitro the root dentin moisture (DM) when soaked in 10% ascorbic acid solution (Group A) and distilled water (Group B) for up to 14 days.

Methods: Forty-four extracted human mandibular single-rooted teeth were sectioned perpendicular to the long axis at the CEJ to access the root dentin. The samples were divided into Groups A and B. Baseline (day 0) DM was measured using a digital grain moisture meter. One sample was placed in each tube, which was then filled with solution. All tubes were kept at 37 degrees C and 100% humidity. DM was measured after 1, 3, 7, and 14 days. The baseline DM value was used as a covariate in the generalized estimating equation (GEE) analysis to account for the difference in the baseline DM between the two groups.

Results: The mean DM (%) values+/-standard deviation on days 0, 1, 3, 7, and 14 were 11.4+/-1.08, 17.1+/-0.87, 18.2+/-0.76, 18.4+/-0.77, and 17.9+/-0.90 in Group A, and 10.2+/-0.95, 12.8+/-0.90, 13.3+/-0.95, 13.0+/-0.91, and 13.2+/-0.89 in Group B, respectively. Group A had significantly higher baseline DM than Group B (p=0.006). After adjusting for differences in the baseline, the GEE analysis indicated that, on average, Group A had a significantly higher increase in DM than Group B, with means+/-standard deviation of 4.35+/-0.26.

Conclusions: The moisture increase in the ascorbic acid group was greater than that in the distilled water group. Soaking root dentin in the unreplenished ascorbic acid solution or distilled water beyond 3 days did not further increase DM.

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Figures

Figure 1
Figure 1
Figure 1a A map of the internal structure of the grain moisture testing machine (TA-5, OGA Electric, Tochigi, Japan) C; capacitor plates, I; impedance measurement Figure 1b The step by step measurement method of the grain moisture testing machine

  1. The picture of the machine,

  2. The root dentin sample was placed on the moisture measuring plate.

  3. The moisture measuring plate was inserted into the machine main body.

  4. The screw chamber was tightened by hand to hold the moisture measuring plate and the root sample.

  5. The button was pressed to measure the moisture.

  6. The moisture was digitally displayed.

See this image and copyright information in PMC

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