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. 2009 Jan;3(1):45-53.
doi: 10.2174/187231209787176353.

Impact of time-dependent inactivation on the estimation of enzyme kinetic parameters for midazolam

Craig Giragossian  1 , Jennifer LaPerle, Rachel E Kosa, Sarah Gillian
Affiliations

Impact of time-dependent inactivation on the estimation of enzyme kinetic parameters for midazolam

Craig Giragossian et al. Drug Metab Lett. 2009 Jan.

Abstract

Enzyme kinetic parameters for midazolam were time-dependent in human liver microsomes, under initial velocity conditions. V(max) and K(m) decreased up to 3.7 and 3.1-fold, respectively, for 10 min compared to 1 min incubations. Mathematical models describing the relationship between inactivation and the time-dependency of enzyme kinetic parameter estimates were derived and discussed.

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