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. 2009 Jun;47(6):1915-7.
doi: 10.1128/JCM.00608-09. Epub 2009 Apr 8.

Validation of five-colony pool analysis using multiplex real-time PCR for detection of diarrheagenic Escherichia coli

F Barletta  1 , T J Ochoa, L Ecker, A I Gil, C F Lanata, T G Cleary
Affiliations

Validation of five-colony pool analysis using multiplex real-time PCR for detection of diarrheagenic Escherichia coli

F Barletta et al. J Clin Microbiol. 2009 Jun.

Abstract

Five Escherichia coli colonies/patient were studied to evaluate the reliability of a multiplex real-time PCR assay for detection of diarrheagenic Escherichia coli groups, using a pool of five colonies rather than individual colonies. Sensitivity and specificity were 98% and 100%, respectively, at a fifth of the cost of the individual colony analysis.

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Figures

FIG. 1.
FIG. 1.
Mixed infection detected in a pool analysis colony showing both an EPEC (eae+) and an ETEC (st+ lt+) infection (A) and confirmed by individual colony analyses corresponding to an ETEC (st+ lt+) colony (B) and an EPEC (eae+) colony (C). −d(RFU)/dt, negative derivative of fluorescence (RFU, relative fluorescence units).
See this image and copyright information in PMC

References

    1. Black, R. E., S. S. Morris, and J. Bryce. 2003. Where and why are 10 million children dying every year? Lancet 3612226-2234. - PubMed
    1. Bryce, J., C. Boschi-Pinto, K. Shibuya, R. E. Black, and the WHO Child Health Epidemiology Reference Group. 2005. WHO estimates of the causes of death in children. Lancet 3651147-1152. - PubMed
    1. Cohen, M. B., J. P. Nataro, D. I. Bernstein, J. Hawkins, N. Roberts, and M. A. Staat. 2005. Prevalence of diarrheagenic Escherichia coli in acute childhood enteritis: a prospective controlled study. J. Pediatr. 14654-61. - PubMed
    1. Guion, C. E., T. J. Ochoa, C. M. Walker, F. Barletta, and T. G. Cleary. 2008. Detection of diarrheagenic Escherichia coli by use of melting-curve analysis and real-time multiplex PCR. J. Clin. Microbiol. 461752-1757. - PMC - PubMed
    1. Huicho, L., M. Trelles, and F. Gonzales. 2006. National and sub-national under-five mortality profiles in Peru: a basis for informed policy decisions. BMC Public Health 6173-182. - PMC - PubMed

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