Comment on

• Changing the recognition site of a conjugative relaxase by rational design.

  González-Pérez B, Carballeira JD, Moncalián G, de la Cruz F. González-Pérez B, et al. Biotechnol J. 2009 Apr;4(4):554-7. doi: 10.1002/biot.200800184. Biotechnol J. 2009. PMID: 19039782
• Expression system of CotA-laccase for directed evolution and high-throughput screenings for the oxidation of high-redox potential dyes.

  Brissos V, Pereira L, Munteanu FD, Cavaco-Paulo A, Martins LO. Brissos V, et al. Biotechnol J. 2009 Apr;4(4):558-63. doi: 10.1002/biot.200800248. Biotechnol J. 2009. PMID: 19156728
• Production of glucolipids and specialty fatty acids from sophorolipids by Penicillium decumbens naringinase: Optimization and kinetics.

  Saerens K, Van Bogaert I, Soetaert W, Vandamme E. Saerens K, et al. Biotechnol J. 2009 Apr;4(4):517-24. doi: 10.1002/biot.200800209. Biotechnol J. 2009. PMID: 19194976
• Fluorescence-based analysis of enzymes at the single-molecule level.

  Blank K, De Cremer G, Hofkens J. Blank K, et al. Biotechnol J. 2009 Apr;4(4):465-79. doi: 10.1002/biot.200800262. Biotechnol J. 2009. PMID: 19229885 Review.
• The empirical valence bond as an effective strategy for computer-aided enzyme design.

  Vardi-Kilshtain A, Roca M, Warshel A. Vardi-Kilshtain A, et al. Biotechnol J. 2009 Apr;4(4):495-500. doi: 10.1002/biot.200800299. Biotechnol J. 2009. PMID: 19229886 Free PMC article.
• Metagenomics: Concept, methodology, ecological inference and recent advances.

  Singh J, Behal A, Singla N, Joshi A, Birbian N, Singh S, Bali V, Batra N. Singh J, et al. Biotechnol J. 2009 Apr;4(4):480-94. doi: 10.1002/biot.200800201. Biotechnol J. 2009. PMID: 19288513 Review.
• A thermostable triple mutant of pyranose 2-oxidase from Trametes multicolor with improved properties for biotechnological applications.

  Spadiut O, Radakovits K, Pisanelli I, Salaheddin C, Yamabhai M, Tan TC, Divne C, Haltrich D. Spadiut O, et al. Biotechnol J. 2009 Apr;4(4):525-34. doi: 10.1002/biot.200800260. Biotechnol J. 2009. PMID: 19291706
• A microreactor for the study of biotransformations by a cross-linked gamma-lactamase enzyme.

  Hickey AM, Ngamsom B, Wiles C, Greenway GM, Watts P, Littlechild JA. Hickey AM, et al. Biotechnol J. 2009 Apr;4(4):510-6. doi: 10.1002/biot.200800302. Biotechnol J. 2009. PMID: 19291707
• Sticky PCR: A PCR-based protocol for targeted protein engineering.

  Yamabhai M. Yamabhai M. Biotechnol J. 2009 Apr;4(4):544-53. doi: 10.1002/biot.200800198. Biotechnol J. 2009. PMID: 19370716
• Directed evolution of a Bacillus chitinase.

  Songsiriritthigul C, Pesatcha P, Eijsink VG, Yamabhai M. Songsiriritthigul C, et al. Biotechnol J. 2009 Apr;4(4):501-9. doi: 10.1002/biot.200800258. Biotechnol J. 2009. PMID: 19370717