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. 2009 Aug 4;137(3):241-5.
doi: 10.1016/j.jconrel.2009.04.015. Epub 2009 Apr 17.

Spatial regulation of specific gene expression through photoactivation of RNAi

Tamaki Endoh  1 , Masahiko Sisido, Takashi Ohtsuki
Affiliations

Spatial regulation of specific gene expression through photoactivation of RNAi

Tamaki Endoh et al. J Control Release. .

Abstract

In this study we describe the spatial regulation of RNA interference (RNAi) using an RNA-carrier protein labeled with a fluorescent dye and a light source to trigger the RNAi. We demonstrate photo-dependent gene silencing using several dyes with different excitation wavelengths. Additionally, we use light from a halogen lamp and a photomask to produce photopatterned RNAi, and laser light to trigger single-cell RNAi on cell culture plates.

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