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. 2009 May;6(5):359-62.
doi: 10.1038/nmeth.1322. Epub 2009 Apr 19.

Universal sample preparation method for proteome analysis

Jacek R Wiśniewski  1 , Alexandre Zougman, Nagarjuna Nagaraj, Matthias Mann
Affiliations

Universal sample preparation method for proteome analysis

Jacek R Wiśniewski et al. Nat Methods. 2009 May.

Abstract

We describe a method, filter-aided sample preparation (FASP), which combines the advantages of in-gel and in-solution digestion for mass spectrometry-based proteomics. We completely solubilized the proteome in sodium dodecyl sulfate, which we then exchanged by urea on a standard filtration device. Peptides eluted after digestion on the filter were pure, allowing single-run analyses of organelles and an unprecedented depth of proteome coverage.

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