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. 2009:536:5-8.
doi: 10.1007/978-1-59745-542-8_2.

Western blotting : remembrance of past things

Affiliations

Western blotting : remembrance of past things

W Neal Burnette. Methods Mol Biol. 2009.

Abstract

Western blotting sprung from the need to develop a sensitive visual assay for the antigen specificity of monoclonal antibodies. The technique employed SDS-PAGE of protein antigens, electrophoretic replica transfer of gel-resolved proteins to unmodified nitrocellulose sheets, probing the immobilized antigens with hybridomas, and detection of antibody-antigen complexes with radiolabeled staphylococcal protein A and autoradiography. The simplicity and relevance of the method has led to its expansive application as an immunodiagnostic and a ubiquitous research tool in biology and medicine.

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