Sending secret messages on nitrocellulose membrane and the use of a molecular pen for orientation in ECL membrane assays

Abstract

Invisible ink and writing secret messages have been part of man's fantasy, having proven useful in clandestine and high sensitivity areas. Security inks, made up of invisible materials that give printed images that cannot be photocopied, that can be read only under special environments have become important. An ink formulation based on silicon (IV) 2,3-naphthalocyanine bis(trihexylsilyloxide) as colorant, invisible to the naked eye but infrared readable, has been described earlier. Biometric DNA ink has also been developed for security authentication. In lighter vein, many budding scientists and others have often experimented with writing secret messages on paper, either for purposes of fun or actually sending secret messages to friends. It involved the use of lemon juice, milk, or other solutions that could be used with a dip pen, brush, or a fountain pen to write invisible messages on a blank white paper. Words turn up as though by magic when the paper is exposed' to heat in one form or the other. Here, an attempt is made to end this book on a slightly humorous note by showing that invisible messages can be written on nitrocellulose membranes (but not on polyvinylidene difluoride membranes) using an appropriately diluted horseradish peroxidase/alkaline phosphatase anti-IgG conjugate (rabbit, mouse, or human anti-IgG). The message is written on the membrane, preferably with a fountain pen, and the membrane is allowed to dry. Regular detection with enhanced chemiluminiscence (ECL) plus or nitro blue tetrazolium/5-bromo-4-chloro-3-indolyl phosphate systems is used to unravel the secret message. In addition, this method could be used to mark nitrocellulose membranes for orientation purposes using ECL detection system and thus can eliminate the use of autoradiography pens.