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. 2009 Jul;53(7):3143-6.
doi: 10.1128/AAC.01473-08. Epub 2009 Apr 20.

First detection of plasmid-encoded blaOXY beta-lactamase

J J González-López  1 , A Coelho, M N Larrosa, S Lavilla, R Bartolomé, G Prats
Affiliations

First detection of plasmid-encoded blaOXY beta-lactamase

J J González-López et al. Antimicrob Agents Chemother. 2009 Jul.

Abstract

Three Klebsiella oxytoca isolates and one Klebsiella pneumoniae isolate from three children admitted to the Hematology Unit of Hospital Vall d'Hebron (Barcelona, Spain) exhibited a susceptibility pattern suggesting OXY beta-lactamase hyperproduction. All the isolates contained a 95-kb plasmid that harbored bla(OXY-1), which was transferred by electrotransformation but could not be self-transferred by conjugation. A qnrS1 gene was also harbored in the bla(OXY-1)-carrying plasmid. This is the first report of a plasmid-encoded OXY beta-lactamase.

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Figures

FIG. 1.
FIG. 1.
(a and b) PFGE analysis of the studied strains digested with S1 endonuclease (a) and Southern blot hybridization with a blaOXY-1 probe (b). (c and d) PFGE analysis of the studied strains digested with XbaI (c) and Southern blot hybridization with a blaOXY-1 probe (d). Hybridization bands at approximately 95 kb in panels b and d correspond with the plasmid harboring blaOXY-1. Hybridization bands at the compression zone in panel b and at approximately 330 kb in panel d may correspond with the chromosomally carried blaOXY-1 gene in K. oxytoca strains. Lanes MK, molecular size markers (numbers at left in kb).
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