Preventive effect of onion juice on selenite-induced experimental cataract

Abstract

Purpose: To evaluate the effects of onion juice on sodium-selenite induced cataract formation.

Materials and methods: Thirty-two 10-day-old Wistar-albino rat pups were divided into four equal groups. Group 1 received only subcutaneous saline injection. In Group 2, sodium-selenite (30 nmol / g body weight) was injected subcutaneously. In Group 3, subcutaneous sodium-selenite was injected and one drop 50% diluted fresh juice of crude onion was instilled every 8 h into the right eye for 14 days; the left eye received no treatment. Group 4 rats were similar to those of Group 3, the only difference being that of undiluted fresh juice of crude onion. The development of cataract was assessed. Rat lenses were analyzed for total antioxidant (TA) level, and for activities of glutathione peroxidase (GPX) and superoxide dismutase (SOD).

Results: Both eyes of all rats in Group 1 did not exhibit cataract formation . In Group 2, all rats developed Grade 3 cataract in the lenses of both eyes. The difference in exhibited cataract in the lens of the right eyes in all rats between Group 2 and any eyes of groups 3 or 4 were significant ( P = 0.001). The mean TA level and mean activities of SOD and GPX in Group 2 rat lenses were significantly lower than the values in lenses of all rats in Group 1 ( P = 0.001, 0.003, 0.001), and in the lenses of the right eyes of rats in Groups 3 and 4 ( P = 0.001, 0.020, 0.001).

Conclusion: Instillation of onion juice into the rat eyes can effectively prevent selenite-induced cataract formation. This effect was associated with increased TA level, SOD and GPX activities in the lens.

Figure 1

Topcon photo-slit-lamp images of rats’ eyes 14 days after injection (a) Clear lens in control group (b) Grade 3 cataract in sodium-selenite group (c) and (d) Clear lens in sodium-selenite + 50% diluted onion juice and sodium-selenite + onion juice groups

Figure 2

Mean TA level in both eyes of rats 14 days after injection in four experimental groups (* = As compared with Se group P = 0.001, ** = As compared with Se group P = 0.248, + = As compared with Se group P = 0.001, ++ = As compared with Se group P = 0.001, TA = Total antioxidant, Se = Selenite)

Figure 3

Mean SOD activity in both eyes of rats 14 days after injection in four experimental groups (* = As compared with Se group P = 0.003, ** = As compared with Se group P = 0.097, + = As compared with Se group P = 0.018, ++ = As compared with Se group P = 0.020, SOD = Superoxide dismutase, Se = Selenite)

Figure 4

Mean GPX activity in both eyes of rats 14 days after injection in four experimental groups (* = As compared with Se group P = 0.001, ** = As compared with Se group P = 0.086, + = As compared with Se group P = 0.001, ++ = As compared with Se group P = 0.001, GPX = Glutathione peroxidase, Se = Selenite)