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. 2009 Jun;238(6):1613-6.
doi: 10.1002/dvdy.21959.

Expression of stem cell pluripotency factors during regeneration in newts

Nobuyasu Maki  1 , Rinako Suetsugu-Maki, Hiroshi Tarui, Kiyokazu Agata, Katia Del Rio-Tsonis, Panagiotis A Tsonis
Affiliations

Expression of stem cell pluripotency factors during regeneration in newts

Nobuyasu Maki et al. Dev Dyn. 2009 Jun.

Abstract

In this study, we present data indicating that mammalian stem cell pluripotency-inducing factors are expressed during lens and limb regeneration in newts. The apparent expression even in intact tissues and the ensued regulation during regeneration raises the possibility that these factors might regulate tissue-specific reprogramming and regeneration. Furthermore, these factors should enable us to understand the similarities and differences between animal regeneration in the newt and stem cell strategies in mammals. Developmental Dynamics 238:1613-1616, 2009. (c) 2009 Wiley-Liss, Inc.

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Figures

Fig. 1
Fig. 1
Phylogenetic tree analysis of all cloned cDNAs, indicating their identity with orthologs from other species.
Fig. 2
Fig. 2
Expression of Oct-4 and nanog detected via PCR in newt ovaries. The expected size for Oct4 was 139bp and for nanog 237bp
Fig. 3
Fig. 3
qPCR analysis of expression of Sox2, Klf4 and c-myc during lens regeneration. For each time point we collected 20 irises, that were pooled for the analysis.
Fig. 4
Fig. 4
qPCR analysis of expression of Sox2, Klf4 and c-myc during regenerating limb blastema formation. For each time point we selected 6 limb stumps or blastemas that were pooled for the analysis.
See this image and copyright information in PMC

References

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