Live and heat-killed Lactobacillus rhamnosus GG: effects on proinflammatory and anti-inflammatory cytokines/chemokines in gastrostomy-fed infant rats
- PMID: 19390478
- DOI: 10.1203/PDR.0b013e3181aabd4f
Live and heat-killed Lactobacillus rhamnosus GG: effects on proinflammatory and anti-inflammatory cytokines/chemokines in gastrostomy-fed infant rats
Abstract
Lactobacillus rhamnosus GG (LGG), a probiotics, ameliorates intestinal and other organ inflammation in infant rats. The hypothesis is that live and heat-killed LGG have similar effects on decreasing the inflammatory response induced by E. coli lipopolysaccharide (LPS) in the infant rat. Using a gastrostomy-fed rat model, 7-d-old rat pups were gastrostomy fed with or without live LGG (10(8) or 10(12) cfu x L(-1) x kg(-1) x d(-1)) for 6 d. In a separate experiment, LPS was administered to rat pups with or without live or heat-killed LGG (10(8) cfu x L(-1) x kg(-1) x d(-1)). Cytokine/chemokine proteins were determined by ELISA or multiplex assay. Both live and heat-killed LGG decreased LPS-induced cytokine-induced neutrophil chemoattractant-1 (CINC-1) production in liver and plasma (p < 0.05) and also showed a trend (p = 0.09) in lungs. Live and heat-killed LGG ameliorated LPS-suppressed IL-10 level in lungs (p < 0.05). Both forms of LGG decreased IL-1b production in liver. There was no difference between low and high doses of live LGG in the production of CINC-1, TNF-alpha, and myeloperoxidase (MPO). There was a trend of increase of claudin-1 in both live and heat-killed groups (p = 0.08). In conclusion, both live and heat-killed LGG provided by the enteral route decrease LPS-induced proinflammatory mediators and increase anti-inflammatory mediators.
Similar articles
-
Lactobacillus rhamnosus GG decreases lipopolysaccharide-induced systemic inflammation in a gastrostomy-fed infant rat model.J Pediatr Gastroenterol Nutr. 2006 May;42(5):545-52. doi: 10.1097/01.mpg.0000221905.68781.4a. J Pediatr Gastroenterol Nutr. 2006. PMID: 16707979
-
Heat-killed Lactobacillus rhamnosus GG modulates urocortin and cytokine release in primary trophoblast cells.Placenta. 2010 Oct;31(10):867-72. doi: 10.1016/j.placenta.2010.04.007. Epub 2010 Aug 8. Placenta. 2010. PMID: 20696472
-
Lactobacillus rhamnosus GG components, SLP, gDNA and CpG, exert protective effects on mouse macrophages upon lipopolysaccharide challenge.Lett Appl Microbiol. 2020 Feb;70(2):118-127. doi: 10.1111/lam.13255. Epub 2019 Dec 18. Lett Appl Microbiol. 2020. PMID: 31782817
-
Live and heat-killed Lactobacillus rhamnosus GG upregulate gene expression of pro-inflammatory cytokines in 5-fluorouracil-pretreated Caco-2 cells.Support Care Cancer. 2014 Jun;22(6):1647-54. doi: 10.1007/s00520-014-2137-z. Epub 2014 Feb 6. Support Care Cancer. 2014. PMID: 24500789
-
Lactobacillus rhamnosus GG modulates innate signaling pathway and cytokine responses to rotavirus vaccine in intestinal mononuclear cells of gnotobiotic pigs transplanted with human gut microbiota.BMC Microbiol. 2016 Jun 14;16(1):109. doi: 10.1186/s12866-016-0727-2. BMC Microbiol. 2016. PMID: 27301272 Free PMC article.
Cited by
-
Beneficial Effects of Viable and Heat-Inactivated Lactobacillus rhamnosus GG Administration on Oxidative Stress and Inflammation in Diet-Induced NAFLD in Rats.Antioxidants (Basel). 2023 Mar 14;12(3):717. doi: 10.3390/antiox12030717. Antioxidants (Basel). 2023. PMID: 36978965 Free PMC article.
-
Effects of dietary inactivated probiotics on growth performance and immune responses of weaned pigs.J Anim Sci Technol. 2021 May;63(3):520-530. doi: 10.5187/jast.2021.e44. Epub 2021 May 31. J Anim Sci Technol. 2021. PMID: 34189502 Free PMC article.
-
Probiotic Lactobacillus Strains Stimulate the Inflammatory Response and Activate Human Macrophages.J Immunol Res. 2017;2017:4607491. doi: 10.1155/2017/4607491. Epub 2017 Jul 5. J Immunol Res. 2017. PMID: 28758133 Free PMC article.
-
Lactobacillus johnsonii JERA01 activates macrophages and increases Th-1 T cell population in mouse small intestine.PLoS One. 2025 Apr 24;20(4):e0320946. doi: 10.1371/journal.pone.0320946. eCollection 2025. PLoS One. 2025. PMID: 40273138 Free PMC article.
-
Fucosylated oligosaccharides in mother's milk alleviate the effects of caesarean birth on infant gut microbiota.Sci Rep. 2018 Sep 13;8(1):13757. doi: 10.1038/s41598-018-32037-6. Sci Rep. 2018. PMID: 30214024 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources
Molecular Biology Databases
Research Materials
Miscellaneous