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. 1991 Jul 5;567(2):459-64.
doi: 10.1016/0378-4347(91)80152-3.

High-performance liquid chromatographic determination of histamine N-methyltransferase activity

H Fukuda  1 , A Yamatodani, I Imamura, K Maeyama, T Watanabe, H Wada
Affiliations

High-performance liquid chromatographic determination of histamine N-methyltransferase activity

H Fukuda et al. J Chromatogr. .

Abstract

A method for the determination of histamine N-methyltransferase (HMT) activity by high-performance liquid chromatography based on post-column derivatization with omicron-phthalaldehyde is described. The determination involves the separation of the substrate, histamine, from its product. N tau-methylhistamine, using a weak cation exchanger, followed by on-line derivatization of these imidazoleamines with omicron-phthalaldehyde and their detection and quantitation with a fluorimetric detector. This assay method is suitable for the measurement of HMT activity during enzyme purification.

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