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Comparative Study
. 1991 Jun;33(1-2):217-22.
doi: 10.1016/0166-0934(91)90021-q.

Comparison of Immobilon-N membrane and other membranes for the detection of HIV-1 genome in high risk patients using PCR

Y S Lee  1 , M J Collie, L K Leung, M Seidlin, S T Beatrice
Affiliations
Comparative Study

Comparison of Immobilon-N membrane and other membranes for the detection of HIV-1 genome in high risk patients using PCR

Y S Lee et al. J Virol Methods. 1991 Jun.

Abstract

The polymerase chain reaction (PCR) was used for detection of the HIV-1 genome from the peripheral blood lymphocytes of high risk patients. The gag regions of HIV-1 (SK38-SK39) were chosen to amplify viral DNA and the amplified products were spotted onto membrane filters and hybridized with a 32P-labeled SK19 probe. Nitrocellulose, nylon and polyvinylidene difluoride (PVDF) membrane filters were used and compared in dot-blot hybridization. PVDF (Immobilon-N, trade name) filter membranes were demonstrated to be the best membranes on the basis of hybridization data and showed a stronger signal on autoradiograms than the other two types (nitrocellulose and nylon).

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