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Review
. 2009 Apr;15(2):62-9.
doi: 10.1007/s10156-009-0669-9. Epub 2009 Apr 25.

Loop-mediated isothermal amplification (LAMP): a rapid, accurate, and cost-effective diagnostic method for infectious diseases

Yasuyoshi Mori  1 , Tsugunori Notomi
Affiliations
Review

Loop-mediated isothermal amplification (LAMP): a rapid, accurate, and cost-effective diagnostic method for infectious diseases

Yasuyoshi Mori et al. J Infect Chemother. 2009 Apr.

Abstract

Loop-mediated isothermal amplification (LAMP) is an established nucleic acid amplification method offering rapid, accurate, and cost-effective diagnosis of infectious diseases. This technology has been developed into commercially available detection kits for a variety of pathogens including bacteria and viruses. The current focus on LAMP methodology is as a diagnostic system to be employed in resource-limited laboratories in developing countries, where many fatal tropical diseases are endemic. The combination of LAMP and novel microfluidic technologies such as Lab-on-a-chip may facilitate the realization of genetic point-of-care testing systems to be used by both developed and developing countries in the near future. This review will describe the historical, current, and future developments of such technologies.

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References

    1. Cohen M.L. Changing patterns of infectious disease. Nature. 2000;406:762–767. 10.1038/35021206. - PubMed
    1. Urdea M., Penny L.A., Olmsted S.S., Giovanni M.Y., Kaspar P., Shepherd A., et al. Requirements for high impact diagnostics in the developing world. Nature. 2006;444:73–79. 10.1038/nature05448. - PubMed
    1. Poxton I.R. Molecular techniques in the diagnosis and management of infectious diseases: do they have a role in bacteriology? Med Princ Pract 14 Suppl. 2005;1:20–26. 10.1159/000086181. - PubMed
    1. Gilbert G.L. Molecular diagnostics in infectious diseases and public health microbiology: cottage industry to postgenomics. Trends Mol Med. 2002;8:280–287. 10.1016/S1471-4914(02)02349-3. - PubMed
    1. Hanscheid T., Grobusch M.P. How useful is PCR in the diagnosis of malaria? Trends Parasitol. 2002;18:395–398. 10.1016/S1471-4922(02)02348-6. - PubMed

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