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. 2009 Jul;47(7):2307-10.
doi: 10.1128/JCM.00240-09. Epub 2009 Apr 29.

Pyrosequencing analysis of 20 nucleotides of internal transcribed spacer 2 discriminates Candida parapsilosis, Candida metapsilosis, and Candida orthopsilosis

Andrew M Borman  1 , Christopher J Linton, Debra Oliver, Michael D Palmer, Adrien Szekely, Frank C Odds, Elizabeth M Johnson
Affiliations

Pyrosequencing analysis of 20 nucleotides of internal transcribed spacer 2 discriminates Candida parapsilosis, Candida metapsilosis, and Candida orthopsilosis

Andrew M Borman et al. J Clin Microbiol. 2009 Jul.

Abstract

Two new cryptic sister species, Candida orthopsilosis and Candida metapsilosis, were recently identified by consistent DNA sequence differences among several genes within the genetically heterogeneous Candida parapsilosis complex. Here, we present data demonstrating that Pyrosequencing analysis of 20 nucleotides of internal transcribed spacer region 2 rapidly and robustly distinguishes between these three closely related Candida species.

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