Reliability of the Vitek 2 yeast susceptibility test for detection of in vitro resistance to fluconazole and voriconazole in clinical isolates of Candida albicans and Candida glabrata
- PMID: 19403774
- PMCID: PMC2691097
- DOI: 10.1128/JCM.02070-08
Reliability of the Vitek 2 yeast susceptibility test for detection of in vitro resistance to fluconazole and voriconazole in clinical isolates of Candida albicans and Candida glabrata
Abstract
The Vitek 2 yeast susceptibility test was evaluated by testing 122 Candida isolates against fluconazole and voriconazole. Excellent categorical agreement with the CLSI broth microdilution method was observed (97.5% for both the azoles). Moreover, the Vitek 2 system was able to identify all but 2 of 59 investigated fluconazole-resistant organisms.
Similar articles
-
Comparison of the Vitek 2 yeast susceptibility system with CLSI microdilution for antifungal susceptibility testing of fluconazole and voriconazole against Candida spp., using new clinical breakpoints and epidemiological cutoff values.Diagn Microbiol Infect Dis. 2013 Sep;77(1):37-40. doi: 10.1016/j.diagmicrobio.2013.05.019. Epub 2013 Jul 16. Diagn Microbiol Infect Dis. 2013. PMID: 23870164
-
Antifungal susceptibility of 205 Candida spp. isolated primarily during invasive Candidiasis and comparison of the Vitek 2 system with the CLSI broth microdilution and Etest methods.J Clin Microbiol. 2010 Jan;48(1):154-61. doi: 10.1128/JCM.01096-09. Epub 2009 Nov 4. J Clin Microbiol. 2010. PMID: 19889902 Free PMC article.
-
Activity of Isavuconazole and Other Azoles against Candida Clinical Isolates and Yeast Model Systems with Known Azole Resistance Mechanisms.Antimicrob Agents Chemother. 2015 Oct 19;60(1):229-38. doi: 10.1128/AAC.02157-15. Print 2016 Jan. Antimicrob Agents Chemother. 2015. PMID: 26482310 Free PMC article.
-
Candida and candidaemia. Susceptibility and epidemiology.Dan Med J. 2013 Nov;60(11):B4698. Dan Med J. 2013. PMID: 24192246 Review.
-
What 'Omics can tell us about antifungal adaptation.FEMS Yeast Res. 2022 Jan 11;21(8):foab070. doi: 10.1093/femsyr/foab070. FEMS Yeast Res. 2022. PMID: 34958354 Free PMC article. Review.
Cited by
-
Multicenter evaluation of the new Vitek 2 yeast susceptibility test using new CLSI clinical breakpoints for fluconazole.J Clin Microbiol. 2014 Jun;52(6):2126-30. doi: 10.1128/JCM.00658-14. Epub 2014 Apr 9. J Clin Microbiol. 2014. PMID: 24719450 Free PMC article.
-
Can We Improve Antifungal Susceptibility Testing?Front Cell Infect Microbiol. 2021 Sep 10;11:720609. doi: 10.3389/fcimb.2021.720609. eCollection 2021. Front Cell Infect Microbiol. 2021. PMID: 34568095 Free PMC article. Review.
-
Machine Learning Approach for Candida albicans Fluconazole Resistance Detection Using Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry.Front Microbiol. 2020 Jan 14;10:3000. doi: 10.3389/fmicb.2019.03000. eCollection 2019. Front Microbiol. 2020. PMID: 32010083 Free PMC article.
-
New Data on the In Vitro Activity of Fenticonazole against Fluconazole-Resistant Candida Species.Antimicrob Agents Chemother. 2020 Nov 17;64(12):e01459-20. doi: 10.1128/AAC.01459-20. Print 2020 Nov 17. Antimicrob Agents Chemother. 2020. PMID: 32988826 Free PMC article. No abstract available.
-
Comparison of the Vitek 2 antifungal susceptibility system with the clinical and laboratory standards institute (CLSI) and European Committee on Antimicrobial Susceptibility Testing (EUCAST) Broth Microdilution Reference Methods and with the Sensititre YeastOne and Etest techniques for in vitro detection of antifungal resistance in yeast isolates.J Clin Microbiol. 2010 May;48(5):1782-6. doi: 10.1128/JCM.02316-09. Epub 2010 Mar 10. J Clin Microbiol. 2010. PMID: 20220169 Free PMC article.
References
-
- Alexander, B. D., T. C. Byrne, K. L. Smith, K. E. Hanson, K. J. Anstrom, J. R. Perfect, and L. B. Reller. 2007. Comparative evaluation of Etest and Sensititre Yeastone panels against the Clinical and Laboratory Standards Institute M27-A2 reference broth microdilution method for testing Candida susceptibility to seven antifungal agents. J. Clin. Microbiol. 45698-706. - PMC - PubMed
-
- Angiolella, L., A. R. Stringaro, F. De Bernardis, B. Posteraro, M. Bonito, L. Toccacieli, A. Torosantucci, M. Colone, M. Sanguinetti, A. Cassone, and A. T. Palamara. 2008. Increase of virulence and its phenotypic traits in drug-resistant strains of Candida albicans. Antimicrob. Agents Chemother. 52:927-936. - PMC - PubMed
-
- Arthington-Skaggs, B. A., W. Lee-Yong, M. A. Ciblak, J. P. Frade, M. E. Brandt, R. A. Hajjeh, L. E. Harrison, A. N. Sofair, and D. W. Warnock. 2002. Comparison of visual and spectrophotometric methods of broth microdilution MIC endpoint determination and evaluation of sterol quantitation method for in vitro susceptibility testing of fluconazole and itraconazole against trailing and non-trailing Candida isolates. Antimicrob. Agents Chemother. 462477-2481. - PMC - PubMed
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources