Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2009 Sep;160(1-2):85-9.
doi: 10.1016/j.jviromet.2009.04.025. Epub 2009 May 3.

Differential diagnosis of orf viruses by a single-step PCR

Kun-Wei Chan  1 , Wei-Li Hsu, Chi-Young Wang, Cheng-Hsiung Yang, Fong-Yuan Lin, Songkhla Chulakasian, Min-Liang Wong
Affiliations

Differential diagnosis of orf viruses by a single-step PCR

Kun-Wei Chan et al. J Virol Methods. 2009 Sep.

Abstract

The complete nucleotide sequence of the A32L gene (named after vaccinia virus, corresponding with open reading frame 108 of the orf virus and encoding an ATPase) of the orf virus was studied using samples of orf virus from infected goats, which were collected from six outbreaks in central Taiwan. DNA sequence analysis of the A32L genes of these and isolates from other countries showed sequence heterogeneity (base pair variation and deletion) in the 3'-terminal regions. This finding led to the development of a polymerase chain reaction (PCR) method for the rapid differential diagnosis of orf virus infections, and the results demonstrated that this was an easy and reliable method for genotyping of orf viruses.

PubMed Disclaimer

Publication types

MeSH terms

Associated data

LinkOut - more resources