NMR assignment of polymerase beta labeled with 2H, 13C, and 15N in complex with substrate DNA
- PMID: 19421423
- PMCID: PMC2676717
- DOI: 10.1007/s12104-007-9007-2
NMR assignment of polymerase beta labeled with 2H, 13C, and 15N in complex with substrate DNA
Abstract
DNA polymerase beta is a multifunctional enzyme involved in base excision repair of nuclear DNA invertebrate cells. We present here the first assignments of the full-length protein (335 residues, 39 kDa) in the presence of a double gap--double hairpin DNA (22 nucleotides, 7 kDa).
Keywords: Base Excision Repair; Polymeraseβ.
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