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. 1977 Apr;4(4):1001-14.
doi: 10.1093/nar/4.4.1001.

Nucleotide sequence of the restriction fragment Hind F-Eco RI2 of SV40 DNA

R Contreras, G Volckaert, F Thys, A Van de Voorde, W Fiers
Free PMC article

Nucleotide sequence of the restriction fragment Hind F-Eco RI2 of SV40 DNA

R Contreras et al. Nucleic Acids Res. 1977 Apr.
Free PMC article

Abstract

The nucleotide sequence of the SV40 genome region between the Hind K fragment and the Eco RI cleavage site has been determined by a combination of three different approaches : analysis of RNA products obtained by transcription with Escherichia coli DNA dependent RNA polymerase, partial degradations with snake venom exonuclease and base-specific chemical degradation of 5'-terminal labeled restriction fragments. This nucleotide sequence shows only one open reading frame and allows the deduction of a small segment of the amino acid sequence of VP1, the major structural protein.

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