Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1977 Apr;4(4):1015-24.
doi: 10.1093/nar/4.4.1015.

Nucleotide sequence of the SV40 DNA restriction fragment Hind C-Hap 2

H Van Heuverswyn, A Van de Voorde, W Fiers
Free PMC article

Nucleotide sequence of the SV40 DNA restriction fragment Hind C-Hap 2

H Van Heuverswyn et al. Nucleic Acids Res. 1977 Apr.
Free PMC article

Abstract

We here report the nucleotide sequence of the SV40 DNA fragment Hind C - Hap 2. The fragment was labeled at the 5'-ends by means of polynucleotide kinase and gamma-(32P) ATP and digested with a suitable restriction enzyme. The separated products were then partially degraded with the base-specific reagents dimethyl-sulphate or hydrazine followed by direct analysis on polyacrylamide gel. The Hind C - Hap 2 sequence is 126 base pairs long and one of the three possible reading frames for translation does not contain any termination codon. So, although no protein is known to be encoded by this region, the possibility cannot yet be completely ruled out. The sequence also contains several AT-rich blocks.

PubMed Disclaimer

References

    1. Virology. 1975 Nov;68(1):260-5 - PubMed
    1. J Mol Biol. 1975 Oct 15;98(1):1-15 - PubMed
    1. Eur J Biochem. 1976 Jan 2;61(1):101-17 - PubMed
    1. Eur J Biochem. 1976 Jan 2;61(1):119-38 - PubMed
    1. J Virol. 1976 May;18(2):664-71 - PubMed