In vitro study of microbial leakage in roots filled with EndoREZ sealer/EndoREZ Points and AH Plus sealer/conventional gutta-percha points

Abstract

Objective: The aim of this study was to determine and compare the microbial leakage of roots filled with EndoREZ sealer/EndoREZ Points and AH Plus sealer/ conventional gutta-percha points.

Materials and methods: 60 single-rooted teeth were prepared using step-back technique. The smear layer was removed with 18% EDTA. Teeth were divided into two experimental groups (n=25 each group) and two controls (n=5 each group). In AH Plus group root canals were obturated with AH Plus sealer/gutta-percha and in EndoREZ group with EndoREZ sealer/EndoREZ Points. Five teeth were served as negative controls and five as positive controls. All teeth were inserted into Eppendorf plastic tubes and suspended in glass bottles containing sterile Schaedler broth. The coronal chambers were filled with the mix of human saliva and broth (ratio 3:1). The medium was changed every 7 days. Microbial growth in the broth was evaluated every day up to the end of experiment.

Results: Leakage in the root canals of the teeth from experimental groups occurred between 4 and 75 days. The mean leakage in AH Plus group was 18.86 days, while in EndoREZ group it was 28.28. No statistically significant difference in microbial leakage between two tested filling materials was found.

Conclusion: Both types of root fillings - EndoREZ sealer/EndoREZ Points and AH Plus sealer/gutta-percha points - showed microbial leakage.