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Comment
. 2009 May;27(5):450-1.
doi: 10.1038/nbt0509-450.

Overpowering the component problem

Matthew R Bennett, Jeff Hasty
Comment

Overpowering the component problem

Matthew R Bennett et al. Nat Biotechnol. 2009 May.
No abstract available

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Figures

Figure 1
Figure 1
Design and redesign of a synthetic gene network. In this example, the researcher wishes to create an intermediate signaling gene between upstream and downstream transcriptional networks. Initially, tTA is used to upregulate the downstream network. tTA is placed under the control of the PLac promoter, which is repressed by LacI produced upstream. However, the Lac promoter is too weak to produce the intended signal. (a) One possible solution is to replace the Lac promoter with a stronger promoter. Because of the lack of available transcription factor–promoter combinations, the best option is the repressible Tet promoter. This requires changes not just in the intermediate gene, but also in the upstream and downstream components (red indicates changed components). (b) With a library of Lac promoters of varying strengths, only one component must be changed to produce the desired output.
See this image and copyright information in PMC

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References

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