Injection of day 2 embryo culture supernatant into the uterine cavity did not improve the pregnancy rate of day 3 embryo transfer in patients who underwent in vitro fertilization-embryo transfer: a randomized clinical trial

Abstract

Objective: To evaluate the effectiveness of uterine cavity injection of day 2 embryo culture supernatant before day 3 embryo transfer in patients who are undergoing in vitro fertilization-embryo transfer.

Design: Randomized controlled trial.

Setting: A reproductive medical centre.

Patient(s): A total of 90 infertile women, 45 of them in the study group and 45 in the control group.

Intervention(s): Uterine cavity infection of day 2 embryo culture supernatant before day 3 embryo transfer.

Main outcome measure(s): Embryo implantation rate and pregnancy rate.

Result(s): The pregnancy versus implantation rates in the study group and control group were 48.9% versus 44.4% and 27.3% versus 22.1%, respectively. Although both pregnancy and implantation rates in the study group were higher than those in the control group, no statistically significant differences were found in these two parameters.

Conclusion(s): Injection of day 2 embryo culture supernatant into the uterine cavity cannot improve the implantation and pregnancy rates of day 3 embryo transfer.