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. 2006 Mar;1(1):53-59.
doi: 10.1007/s11468-006-9006-7. Epub 2006 Mar 1.

Microwave-Accelerated and Metal-Enhanced Fluorescence Myoglobin Detection on Silvered Surfaces: Potential Application to Myocardial Infarction Diagnosis

Kadir Aslan, Chris D Geddes

Microwave-Accelerated and Metal-Enhanced Fluorescence Myoglobin Detection on Silvered Surfaces: Potential Application to Myocardial Infarction Diagnosis

Kadir Aslan et al. Plasmonics. 2006 Mar.

Abstract

In this short paper, we describe a novel approach to both significantly accelerate and optically amplify fluorescence-based immunoassays. Our approach utilizes metal-enhanced fluorescence (MEF) to intrinsically optically amplify fluorescence signatures, which, when combined with the use of low-power microwaves to kinetically accelerate assays, provides for both ultrafast and ultrabright immunoassays. Surprisingly, the use of low-power microwaves and silver nanostructures provides for localized heating, concentrating the effect to the particles themselves as compared to the generic heating of the high dielectric assay fluid. We have subsequently applied our microwave-accelerated MEF approach to the detection of myoglobin, where its rapid quantification is paramount for the clinical assessment of an acute myocardial infarction.

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Figures

Figure 1
Figure 1
Microwave-accelerated metal-enhanced fluorescence myoglobin immunoassay.
Figure 2
Figure 2
Total-internal reflection fluorescence geometry for myoglobin assessment.
Figure 3
Figure 3
Absorption spectrum of silver-island films (SiFs) both before and after exposure to low-power microwaves.
Figure 4
Figure 4
Emission spectrum of Alexa 647 on both SiFs and glass after 30-min incubation (top) and normalized to the fluorescence emission on glass (bottom). The spectra are the mean of five measurements on the respective surfaces.
Figure 5
Figure 5
Emission spectrum of Alexa 647 on both SiFs and glass after 20-s low-power microwave heating (top) and normalized to the fluorescence emission on glass (bottom). The spectra are the mean of five measurements on the respective surfaces.
Figure 6
Figure 6
Control experiments: emission spectrum of Alexa 647 on both SiFs and glass after 30-s incubation at room temperature (with myoglobin; top), after 30-min incubation at room temperature (no myoglobin; middle), and after 30-s low-power microwave heating (no myoglobin; bottom).
Figure 7
Figure 7
Emission intensity of Alexa 647 on both SiFs and glass after cumulative low-power microwave heating. λex = 650 nm.
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