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. 2009;48(25):4524-7.
doi: 10.1002/anie.200805683.

Efficient replication bypass of size-expanded DNA base pairs in bacterial cells

James C Delaney  1 , Jianmin Gao, Haibo Liu, Nidhi Shrivastav, John M Essigmann, Eric T Kool
Affiliations

Efficient replication bypass of size-expanded DNA base pairs in bacterial cells

James C Delaney et al. Angew Chem Int Ed Engl. 2009.

Abstract

Supersize me! Size-expanded DNA bases (xDNA) are able to encode natural DNA sequences in replication. In vitro experiments with a DNA polymerase show nucleotide incorporation opposite the xDNA bases with correct pairing. In vivo experiments using E. coli show that two xDNA bases (xA and xC, see picture) encode the correct replication partners.

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Figures

Figure 1
Figure 1
Structures and DNA sequence in this study. A. Structures of the four xDNA bases evaluated in cellular replication, with their paired structures shown. B. DNA sequence context for xDNA bases (at position X) inserted into single-stranded M13 bacteriophage.
Figure 2
Figure 2
The efficiency of replication bypass of single xDNA bases in vivo. Bases were ligated into single-stranded M13 bacteriophage and replicated in E. coli. Bypass efficiency was measured by quantifying relative output signals from test and internal standard genomes, and normalizing to those from the G at the test site control.
Figure 3
Figure 3
The replicative encoding capability of single xDNA bases in E. coli. Shown are the relative amounts of each natural base at the test site (X) that replaced the xDNA base shown after phage replication.
See this image and copyright information in PMC

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