Prolactin short-loop feedback and prolactin inhibition of luteinizing hormone secretion during the breeding season and seasonal anoestrus in the ewe

Abstract

In seasonally breeding mammals, plasma prolactin (PRL) concentrations vary on an annual basis with levels high in summer and low in winter. In this study of the ewe, we determined, first, whether PRL secretion is regulated by short-loop feedback and, second, whether the high summer levels of PRL are due to a change in sensitivity or loss of this feedback loop. Because the high summer levels of PRL coincide with the period of seasonal anoestrus in the ewe and could therefore be involved in the seasonal suppression of gonadotrophins, the effects of intracerebroventricular PRL on pulsatile LH secretion were also determined. Ovary intact ewes received intracerebroventricular injections of ovine PRL (oPRL; 50 micrograms) or anti-PRL serum. From 3 to 13 h after central administration of oPRL, plasma PRL concentrations were significantly reduced compared with the vehicle-treated controls. In contrast, following injection of anti-PRL serum, plasma PRL levels increased significantly. To determine whether there was a seasonal change in sensitivity to PRL feedback, a series of experiments were conducted in July and November when PRL concentrations are high and low, respectively. At each time of year, ovariectomized oestradiol-implanted ewes were injected intracerebroventricularly with 10 and 50 micrograms oPRL with control animals receiving the vehicle. At both times of year there was clear evidence of PRL short-loop feedback with no indication that sensitivity was reduced in the July trial. Luteinizing hormone (LH) pulse frequency, pulse amplitude and mean LH were not affected by intracerebroventricular oPRL at either time of year.(ABSTRACT TRUNCATED AT 250 WORDS)