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. 2009 Aug;166(2):153-8.
doi: 10.1016/j.molbiopara.2009.03.011. Epub 2009 Apr 9.

Hundreds of microsatellites for genotyping Plasmodium yoelii parasites

Jian Li  1 , Yanhui Zhang, Shengfa Liu, Lingxian Hong, Margery Sullivan, Thomas F McCutchan, Jane M Carlton, Xin-zhuan Su
Affiliations

Hundreds of microsatellites for genotyping Plasmodium yoelii parasites

Jian Li et al. Mol Biochem Parasitol. 2009 Aug.

Abstract

Genetic crosses have been employed to study various traits of rodent malaria parasites and to locate loci that contribute to drug resistance, immune protection, and disease virulence. Compared with human malaria parasites, genetic crossing of rodent malaria parasites is more easily performed; however, genotyping methods using microsatellites (MSs) or large-scale single nucleotide polymorphisms (SNPs) that have been widely used in typing Plasmodium falciparum are not available for rodent malaria species. Here we report a genome-wide search of the Plasmodium yoelii yoelii (P. yoelii) genome for simple sequence repeats (SSRs) and the identification of nearly 600 polymorphic MS markers for typing the genomes of P. yoelii and Plasmodium berghei. The MS markers are randomly distributed across the 14 physical chromosomes assembled from genome sequences of three rodent malaria species, although some variations in the numbers of MS expected according to chromosome size exist. The majority of the MS markers are AT-rich repeats, similar to those found in the P. falciparum genome. The MS markers provide an important resource for genotyping, lay a foundation for developing linkage maps, and will greatly facilitate genetic studies of P. yoelii.

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Figures

Fig. 1
Fig. 1
Distribution of Plasmodium yoelii polymorphic microsatellite markers on the rodent malaria composite chromosome maps. The markers were assigned to composite chromosome maps of rodent malaria parasites described by Kooij et al. [15]. The chromosomes are as marked from 1 to 14; the vertical ticks on top of the chromosome lines indicate the position of microsatellites.
Fig. 2
Fig. 2
Relationship of the number of microsatellite (MS) on each chromosome and the chromosome length. Total numbers of nucleotide numbers (base pairs) from all the contigs assigned to a chromosome were plotted against the numbers of MS markers identified. The numbers inside each circle indicate each of the 14 chromosomes. R2 is the correlation coefficiency for the regression line.
See this image and copyright information in PMC

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