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. 1977 May 1;163(2):261-7.
doi: 10.1042/bj1630261.

Some properties of aldehyde dehydrogenase from sheep liver mitochondria

G J Hart, F M Dickinson

Some properties of aldehyde dehydrogenase from sheep liver mitochondria

G J Hart et al. Biochem J. .

Abstract

Aldehyde dehydrogenase from sheep liver mitochondria was purified to homogeneity as judged by electrophoresis on polyacrylamide gels, and by sedimentation-equilibrium experiments in the analytical ultracentrifuge. The enzyme has a molecular weight of 198000 and a subunit size of 48000, indicating that the molecule is a tetramer. Fluorescence and spectrophotometric titrations indicate that each subunit can bind 1 molecule of NADH. Enzymic activity is completely blocked by reaction of 4mol of 5,5'-dithiobis-(2-nitrobenzoate)/mol of enzyme. Excess of disulfiram or iodoacetamide decreases activity to only 50% of the control value, and only two thiol groups per molecule are apparently modified by these reagents.

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