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. 1991 Oct 25;19(20):5613-7.
doi: 10.1093/nar/19.20.5613.

Human Alu subfamilies and their methylation revealed by blot hybridization

C W Schmid  1
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Free PMC article

Human Alu subfamilies and their methylation revealed by blot hybridization

C W Schmid. Nucleic Acids Res. .
Free PMC article

Abstract

By a simple direct blot hybridization strategy, the existence of human Alu family subfamilies is confirmed. Using consensus restriction cleavage sites, individual bands can be resolved from genomic human DNA digests corresponding to three distinct Alu subfamilies. Digestion with methylation sensitive and insensitive restriction enzymes shows that the numerous CpG residues in the youngest Alu subfamilies are largely methylated in vivo, suggesting a model for the transcriptional regulation of Alu repeats.

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References

    1. Nucleic Acids Res. 1980 Apr 11;8(7):1499-504 - PubMed
    1. J Biol Chem. 1962 Jun;237:1961-7 - PubMed
    1. Nucleic Acids Res. 1987 Nov 25;15(22):9429-49 - PubMed
    1. Mol Biol Evol. 1987 Jan;4(1):19-29 - PubMed
    1. J Mol Evol. 1987;26(3):180-6 - PubMed

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