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. 1991 Nov 11;19(21):5839-42.
doi: 10.1093/nar/19.21.5839.

W (A or T) sequences as probes and primers suitable for genomic mapping and fingerprinting

R Drmanac  1 , D Nizetic, G G Lennon, A Beitverda, H Lehrach
Affiliations
Free PMC article

W (A or T) sequences as probes and primers suitable for genomic mapping and fingerprinting

R Drmanac et al. Nucleic Acids Res. .
Free PMC article

Abstract

A limitation to the use of oligonucleotide probes as tools for genetic and physical mapping has been the low hybridization positive frequency obtained by oligonucleotides of sufficient length to hybridize preferentially to cloned insert DNA (and not host E. coli genomic DNA). Both computer and experimental results now indicate that oligonucleotide probes composed of W (A or T) sequence are preferentially found in eukaryotic DNA, and can be used to provide high frequency, discriminative hybridization. Such W sequences may be useful as either probes or PCR primers in molecular diagnostic applications as well as in genetic and physical mapping.

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References

    1. Proc Natl Acad Sci U S A. 1990 Aug;87(16):6218-22 - PubMed
    1. Biochim Biophys Acta. 1990 May 24;1049(1):21-6 - PubMed
    1. Nucleic Acids Res. 1990 Nov 25;18(22):6531-5 - PubMed
    1. Nucleic Acids Res. 1991 Jan 11;19(1):182 - PubMed
    1. Proc Natl Acad Sci U S A. 1991 Apr 15;88(8):3233-7 - PubMed