Lateralization of the connections of the ovary to the celiac ganglia in juvenile rats

Abstract

During the development of the female rat, a maturing process of the factors that regulate the functioning of the ovaries takes place, resulting in different responses according to the age of the animal. Studies show that peripheral innervation is one relevant factor involved.In the present study we analyzed the anatomical relationship between the neurons in the celiac-superior mesenteric ganglia (CSMG), and the right or left ovary in 24 or 28 days old female pre-pubertal rats. The participation of the superior ovarian nerve (SON) in the communication between the CSMG and the ovaries was analyzed in animals with unilateral section of the SON, previous to injecting true blue (TB) into the ovarian bursa. The animals were killed seven days after treatment. TB stained neurons were quantified at the superior mesenteric-celiac ganglia.The number of labeled neurons in the CSMG of rats treated at 28 days of age was significantly higher than those treated on day 24. At age 24 days, injecting TB into the right ovary resulted in neuron stains on both sides of the celiac ganglia; whereas, injecting the left side the stains were exclusively ipsilateral. Such asymmetry was not observed when the rats were treated at age of 28 days.In younger rats, sectioning the left SON resulted in significantly lower number of stained neurons in the left ganglia while sectioning the right SON did not modify the number of stained neurons. When sectioning of the SON was performed to 28 days old rats, no staining was observed.Present results show that the number and connectivity of post-ganglionic neurons of the CSMG connected to the ovary of juvenile female rats change as the animal mature; that the SON plays a role in this communication process as puberty approaches; and that this maturing process is different for the right or the left ovary.

Figure 1

The left and right CSMG. 4×.

Figure 2

Number of positive neurons to true blue in the celiac superior-mesenteric ganglia of the animals injected with the true blue in the left or right ovarian bursa at 24 or 28 days old, and sacrificed seven days later. * p < 0.05 vs. contralateral CSMG.

Figure 3

True Blue labeled neurons (white arrows) in the CSMG 24 days old rats injected with TB in the left (a, b) or into the right ovary (c, d). Left CSMG a and c; Right CSMG b and d. Scale bar 100 μm.

Figure 4

True Blue labeled neurons (white arrows) in the CSMG 28 days old rats injected with TB in the left (a, b) or into the right ovary (b, c). Left CSMG a and c; Right CSMG b and d.

Figure 5

Diferences in the intensitity of TB positive cells in the CSMG of 24 and 28 days old rats.

Figure 6

True Bleu labeled neurons (white arrows) in the CSMG of 24 days old rats with section of the left SON injected with TB in the left ovary (a) or with section of the right SON injected with TB into the right ovary (b).