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. 1977 Jun;35(6):785-94.
doi: 10.1038/bjc.1977.120.

Properties of a cell line from human adenocarcinoma of the rectum

J F Watkins, C Sanger
Free PMC article

Properties of a cell line from human adenocarcinoma of the rectum

J F Watkins et al. Br J Cancer. 1977 Jun.
Free PMC article

Abstract

A new, highly differentiated line of cells derived from adenocarcinoma of the rectum (HT55) is described. This line is noteworthy for the following features: 1. The role played in its development by the use of UV-inactivated Sendai virus to attach tumour cell clumps to plastic bottles. 2. Evidence that it produces RNA-containing material of density 1-5--1-16 g/ml. 3. Induction of bone formation in the stroma when grown in athymic mice. 4. Stimulation of primary CBA mouse embryo fibroblasts to form a transient nodule when mixed with them and injected into adult CBA mice. The karyotype and growth-cycle characteristics of the line are described.

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