Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
Review
. 2009;47(6):561-70.
doi: 10.1080/13693780902953886.

Consensus multi-locus sequence typing scheme for Cryptococcus neoformans and Cryptococcus gattii

Wieland Meyer  1 , David M Aanensen, Teun Boekhout, Massimo Cogliati, Mara R Diaz, Maria Carmela Esposto, Matthew Fisher, Felix Gilgado, Ferry Hagen, Sirada Kaocharoen, Anastasia P Litvintseva, Thomas G Mitchell, Sitali P Simwami, Luciana Trilles, Maria Anna Viviani, June Kwon-Chung
Affiliations
Review

Consensus multi-locus sequence typing scheme for Cryptococcus neoformans and Cryptococcus gattii

Wieland Meyer et al. Med Mycol. 2009.

Abstract

This communication describes the consensus multi-locus typing scheme established by the Cryptococcal Working Group I (Genotyping of Cryptococcus neoformans and C. gattii) of the International Society for Human and Animal Mycology (ISHAM) using seven unlinked genetic loci for global strain genotyping. These genetic loci include the housekeeping genes CAP59,GPD1, LAC1, PLB1, SOD1, URA5 and the IGS1 region. Allele and sequence type information are accessible at http://www.mlst.net/ .

PubMed Disclaimer

Conflict of interest statement

Declaration of interest: The authors report no conflicts of interest. The authors alone are responsible for the content and writing of the paper.

Figures

Fig. 1
Fig. 1
Number of genes necessary to differentiate all sequence types obtained in the two previously published cryptococcal MLST schemes based on Simpson’s index of diversity [38]. For the Fraser et al. [28] data set the mating type locus specific genes, SXIa or SXIα, have been excluded for this analysis since they can’t be amplified from all isolates.
Fig. 2
Fig. 2
CAP59 genomic sequence of strain H99 (Cryptococcus neoformans var. grubii, VNI, http://www.broad.mit.edu) used as master sequence, indicating the location of the Cryptococcal Working Group (ISHAM) proposed consensus primers used in Fraser et al. [28] and the location of the primers used in Litvintseva et al. [23].
See this image and copyright information in PMC

References

    1. Evans EE. The antigenic composition of Cryptococcus neoformans. I. A serologic classification by means of the capsular and agglutination reactions. J Immunol. 1950;64:423–430. - PubMed
    1. Kwon-Chung KJ. Morphogenesis of Filobasidiella neoformans, the sexual state of Cryptococcus neoformans. Mycologia. 1976;68:821–833. - PubMed
    1. Kwon-Chung KJ. A new species of Filobasidiella, the sexual state of Cryptococcus neoformans B and C serotypes. Mycologia. 1976;68:943–946. - PubMed
    1. Kwon-Chung KJ, Varma A. Do major species concepts support one, two or more species within Cryptococcus neoformans? FEMS Yeast Res. 2006;6:574–587. - PubMed
    1. Franzot SP, Salkin IF, Casadevall A. Cryptococcus neoformans var. grubii: separate varietal status for Cryptococcus neoformans serotype A isolates. J Clin Microbiol. 1999;37:838–840. - PMC - PubMed

Publication types