[Genotyping of AKAP10 gene 2073A/G single nucleotide polymorphism by TaqMan probe real-time PCR]

Abstract

Objective: To detect AKAP10 gene 2073A/G single nucleotide polymorphism (SNP) genotyping by TaqMan probe real-time PCR.

Methods: The genotype of AKAP10 gene 2073A/G was detected by real-time PCR with a pair of new-designed TaqMan probes. The PCR products also were subjected to gene sequence analysis to validate the results of TaqMan probe real-time PCR.

Results: The TaqMan probe real-time PCR method was successfully developed to detect AKAP10 gene 2073A/G SNP. The results were accordant with those achieved by DNA sequencing. The distribution of AKAP10 gene 2073A/G among population had no relationship with gender, age (P > 0.05). Unconditional logistic regression analysis revealed that the variant genotypes (AG + GG) had a 52% increased risk of colorectal cancer, compared with the AA genotype (P = 0.019).

Conclusion: A detection platform for SNP genotyping by TaqMan probe was set up successfully. There was a significant association between AKAP10 gene 2073A/G and colorectal cancer.