PCR based detection of furadan genotoxicity effects in rohu (Labeo rohita) fingerlings

Abstract

The present study involves the use of RAPD-PCR to evaluate the genotoxic effects of furadan in the DNA of Labeo rohita (rohu) fingerlings. Rohu fingerlings were exposed to 0.02 ppm of furadan for a total period of 96 h and samplings were done at 24, 48, 72 and 96 h. RAPD - PCR were carried out with the blood and liver DNA samples of both control and treated groups at each of the four sampling hours. A total of six selected RAPD primers were used for PCR amplification. Template stability has been taken as the measure of DNA damage caused by pesticide. The results obtained showed no significant difference in the template stability in the blood DNA of furadan treated groups at any of the four sampling hours; however, the liver DNA were able to show significant difference at 48 and 96 hours of treatment.