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Comparative Study
. 2010 Feb;12(1):14-23.
doi: 10.1007/s10126-009-9195-0. Epub 2009 May 28.

The selectivity of milking of Dunaliella salina

Dorinde M M Kleinegris  1 , Marcel Janssen, Willem A Brandenburg, René H Wijffels
Affiliations
Comparative Study

The selectivity of milking of Dunaliella salina

Dorinde M M Kleinegris et al. Mar Biotechnol (NY). 2010 Feb.

Abstract

The process of the simultaneous production and extraction of carotenoids, milking, of Dunaliella salina was studied. We would like to know the selectivity of this process. Could all the carotenoids produced be extracted? And would it be possible to vary the profile of the produced carotenoids and, consequently, influence the type of carotenoids extracted? By using three different D. salina strains and three different stress conditions, we varied the profiles of the carotenoids produced. Between Dunaliella bardawil and D. salina 19/18, no remarkable differences were seen in the extraction profiles, although D. salina 19/18 seemed to be better extractable. D. salina 19/25 was not "milkable" at all. The milking process could only be called selective for secondary carotenoids in case gentle mixing was used. In aerated flat-panel photobioreactors, extraction was much better, but selectiveness decreased and also chlorophyll and primary carotenoids were extracted. This was possibly related to cell damage due to shear stress.

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Figures

Fig. 1
Fig. 1
Growth curves of D. bardawil during cultivation and stress with a hight light intensity, b low temperature, and c the combination of high light intensity and low temperature. Dashed lines indicate start stress. Error bars represent the standard deviation, n = 2
Fig. 2
Fig. 2
a Carotenoids produced by D. bardawil under stress conditions: no stress, HL high light intensity, LT low temperature, HL/LT combination of high light intensity and low temperature. Error bars represent the 95% confidence interval. b Carotenoids produced by D. salina 19/18 under stress conditions: no stress, HL high light intensity, LT low temperature, HL/LT combination of high light intensity and low temperature. Error bars represent the 95% confidence interval. c Carotenoids produced by D. salina 19/25 under stress conditions: no stress, HL high light intensity, LT low temperature, HL/LT combination of high light intensity and low temperature. Error bars represent the 95% confidence interval
Fig. 3
Fig. 3
Extracted carotenoids and chlorophylls as the percentage produced either by D. bardawil, D. salina 19/18, or D. salina 19/25; (db) D. bardawil, (19/18) D. salina 19/18, (19/25) D. salina 19/25. Stress by L high light intensity, T low temperature, or LT combination of high light intensity and low temperature. Error bars represent the standard deviation, n = 3
Fig. 4
Fig. 4
Extracted carotenoids and chlorophylls as percentage produced either by D. bardawil or D. salina 19/18; (db) D. bardawil, (19/18) D. salina 19/18. Stress by L high light intensity, T low temperature, or LT combination of high light intensity and low temperature. Error bars represent the standard deviation, n = 3
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