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. 2004 Sep 6;12(18):4203-13.
doi: 10.1364/opex.12.004203.

Femtosecond laser disruption of subcellular organelles in a living cell

Wataru Watanabe, Naomi Arakawa, Sachihiro Matsunaga, Tsunehito Higashi, Kiichi Fukui, Keisuke Isobe, Kazuyoshi Itoh
Free article

Femtosecond laser disruption of subcellular organelles in a living cell

Wataru Watanabe et al. Opt Express. .
Free article

Abstract

Subcellular organelles in living cells were inactivated by tightly focusing femtosecond laser pulses inside the cells. Photodisruption of a mitochondrion in living cells was experimentally confirmed by stacking three-dimensional confocal images and by restaining of organelles. The viability of the cells after femtosecond laser irradiation was ascertained by impermeability of propidium iodide as well as by the presence of cytoplasmic streaming.

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