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Review
. 2009 Jun:Chapter 17:17.1.1-17.1.15.
doi: 10.1002/0471142700.nc1701s37.

UV Melting of G-Quadruplexes

Jean-Louis Mergny  1 , Laurent Lacroix  1
Affiliations
Review

UV Melting of G-Quadruplexes

Jean-Louis Mergny et al. Curr Protoc Nucleic Acid Chem. 2009 Jun.

Abstract

Absorbance versus temperature curves can provide information on the thermal stability of DNA or RNA quadruplexes. Quadruplex denaturation or renaturation can be followed by recording absorbance at 295 nm rather than 260 nm, the wavelength used to monitor duplex denaturation. This unit describes the use of absorbance versus temperature curves to determine melting temperatures (T(m) values) and model-dependent thermodynamic parameters.

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References

Literature Cited

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Internet Resources
    1. http://www.quadruplex.org

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