Rapid biochemical tests for characterization of the Mycoplasmatales
- PMID: 194917
- PMCID: PMC274645
- DOI: 10.1128/jcm.5.5.531-534.1977
Rapid biochemical tests for characterization of the Mycoplasmatales
Abstract
Methods are described for the rapid detection of beta-D-glucosidase and phosphatase in mycoplasma cultures using fluorogenic 4-methylumbelliferone substrates. These methods were applied to a selection of mycoplasma cultures and were compared with the conventionally used tests for these enzymes. Results were similar by both methods, but the fluorogenic tests could be read after 1 h, whereas the conventional tests took several days.
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