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Comparative Study
. 1977 May;5(5):531-4.
doi: 10.1128/jcm.5.5.531-534.1977.

Rapid biochemical tests for characterization of the Mycoplasmatales

J M Bradbury
Comparative Study

Rapid biochemical tests for characterization of the Mycoplasmatales

J M Bradbury. J Clin Microbiol. 1977 May.

Abstract

Methods are described for the rapid detection of beta-D-glucosidase and phosphatase in mycoplasma cultures using fluorogenic 4-methylumbelliferone substrates. These methods were applied to a selection of mycoplasma cultures and were compared with the conventionally used tests for these enzymes. Results were similar by both methods, but the fluorogenic tests could be read after 1 h, whereas the conventional tests took several days.

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