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. 2009 Aug;47(8):2607-10.
doi: 10.1128/JCM.00770-09. Epub 2009 Jun 3.

Expanded multilocus sequence typing for burkholderia species

Theodore Spilker  1 , Adam Baldwin, Amy Bumford, Chris G Dowson, Eshwar Mahenthiralingam, John J LiPuma
Affiliations

Expanded multilocus sequence typing for burkholderia species

Theodore Spilker et al. J Clin Microbiol. 2009 Aug.

Abstract

PCR primers targeting loci in the current Burkholderia cepacia complex multilocus sequence typing scheme were redesigned to (i) more reliably amplify these loci from B. cepacia complex species, (ii) amplify these same loci from additional Burkholderia species, and (iii) enable the use of a single primer set per locus for both amplification and DNA sequencing.

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Figures

FIG. 1.
FIG. 1.
Phylogenetic tree of concatenated nucleotide sequences from the seven MLST loci, generated by using Clustal V. Burkholderia strains included are representatives of the strain set analyzed as part of this study (see supplemental material) or strains for which genome sequence data were available in public databases (indicated by ‡ symbol). Burkholderia strains in bold type are discussed in the text. Strains belonging to various Burkholderia species and Burkholderia sp. strains are shown.
See this image and copyright information in PMC

References

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