Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
Comparative Study
. 2009 Aug;47(8):2604-6.
doi: 10.1128/JCM.00632-09. Epub 2009 Jun 3.

Assessing human immunodeficiency virus type 1 tropism: Comparison of assays using replication-competent virus versus plasma-derived pseudotyped virions

Noriaki Hosoya  1 , Zhaohui Su, Timothy Wilkin, Roy M Gulick, Charles Flexner, Michael D Hughes, Paul R Skolnik, Françoise Giguel, Wayne L Greaves, Eoin Coakley, Daniel R Kuritzkes
Affiliations
Comparative Study

Assessing human immunodeficiency virus type 1 tropism: Comparison of assays using replication-competent virus versus plasma-derived pseudotyped virions

Noriaki Hosoya et al. J Clin Microbiol. 2009 Aug.

Abstract

Detection of CXCR4-using human immunodeficiency virus by the Trofile assay was compared to that by assays using virus isolates or replication-competent recombinants. Concordance with the Trofile assay was good, but assays using replicating viruses did not increase substantially the ability to detect the presence of CXCR4-using virus.

PubMed Disclaimer

Similar articles

See all similar articles

Cited by

References

    1. Björndal, Å., H. Deng, M. Jansson, J. R. Fiore, C. Colognesi, A. Karlsson, J. Albert, G. Scarlatti, D. R. Littman, and E. M. Fenyo. 1997. Coreceptor usage of primary human immunodeficiency virus type 1 isolates varies according to biological phenotype. J. Virol. 717478-7487. - PMC - PubMed
    1. Demeter, L. M., R. J. Bosch, R. W. Coombs, S. Fiscus, J. Bremer, V. A. Johnson, A. Erice, J. B. Jackson, S. A. Spector, K. M. Squires, M. A. Fischl, M. D. Hughes, and S. M. Hammer. 2002. Detection of replication-competent human immunodeficiency virus type 1 (HIV-1) in cultures from patients with levels of HIV-1 RNA in plasma suppressed to less than 500 or 50 copies per milliliter. J. Clin. Microbiol. 402089-2094. - PMC - PubMed
    1. Fatkenheuer, G., M. Nelson, A. Lazzarin, I. Konourina, A. I. Hoepelman, H. Lampiris, B. Hirschel, P. Tebas, F. Raffi, B. Trottier, N. Bellos, M. Saag, D. A. Cooper, M. Westby, M. Tawadrous, J. F. Sullivan, C. Ridgway, M. W. Dunne, S. Felstead, H. Mayer, and E. van der Ryst. 2008. Subgroup analyses of maraviroc in previously treated R5 HIV-1 infection. N. Engl. J. Med. 3591442-1455. - PubMed
    1. Gulick, R. M., Z. Su, C. Flexner, M. D. Hughes, P. R. Skolnik, T. J. Wilkin, R. Gross, A. Krambrink, E. Coakley, W. L. Greaves, A. Zolopa, R. Reichman, C. Godfrey, M. Hirsch, D. R. Kuritzkes, et al. 2007. Phase 2 study of the safety and efficacy of vicriviroc, a CCR5 inhibitor, in HIV-1-infected, treatment-experienced patients. J. Infect. Dis. 196304-312. - PubMed
    1. Hollinger, F. B., J. W. Bremer, L. E. Myers, J. W. M. Gold, L. McQuay, and The NIH/NIAID/DAIDS/ACTG Virology Laboratories. 1992. Standardization of sensitive human immunodeficiency virus coculture procedures and establishment of a multicenter quality assurance program for the AIDS Clinical Trials Group. J. Clin. Microbiol. 301787-1794. - PMC - PubMed

Publication types

Substances

LinkOut - more resources