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Comparative Study
. 1991 Aug;29(1-2):89-103.
doi: 10.1016/0165-2427(91)90055-h.

Natural killer (NK) activity of porcine blood lymphocytes against allogeneic melanoma target cells

M Büttner  1 , R Wanke, B Obermann
Affiliations
Comparative Study

Natural killer (NK) activity of porcine blood lymphocytes against allogeneic melanoma target cells

M Büttner et al. Vet Immunol Immunopathol. 1991 Aug.

Abstract

Allogeneic PM/86 melanoma cells of Munich Troll miniature swine have been used for the demonstration of porcine peripheral blood NK cell activity. Compared with the specific lysis of xenogeneic K562-, U937- and Vero-target cells, NK cell-mediated cytotoxicity (NK-CMC) against PM/86 melanoma tumor cells was significantly lower in a 16 h chromium release assay. The target cell susceptibility to peripheral blood NK-CMC of both adult Troll miniature swine and German Landrace sows was very similar. Cold target inhibition assays revealed the allogeneic PM/86 melanoma cells to be the most powerful inhibitors of NK-CMC. Nylon wool non-adherent lymphocytes produced interferon (IFN)-alpha in different quantities upon contact with NK susceptible target cells. The NK effector cells could be stimulated to a higher lytic activity against all susceptible targets by a moderate dose of natural human interleukin-2 (nhuIL-2). The role of NK-CMC in melanoma tumor rejection and/or prevention of metastases is yet unknown in swine although porcine melanoma serves as a good model for the disease in man.

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References

    1. Anderson L., Nilsson K., Gohmberg C. K562-a erythroleukemic cell line. Int. J. Cancer. 1979;23:143–147. - PubMed
    1. Beattie C.W., Tissot R., Amoss M. Experimental models in human melanoma research: a logistic perspective. Sem. Oncol. 1988;15:500–511. - PubMed
    1. Berkelhammer J., Ensing B.M., Hook R.R. Growth and spontaneous regression of swine melanoma: relationship of in vitro leukocyte reactivity. J. Nat. Cancer Inst. 1982;68:461–468. - PubMed
    1. Bhagyam R.C., Jarrett-Zaczek D., Ferguson F.G. Activation of swine peripheral blood lymphocytes with human recombinant interleukin-2. Immunology. 1988;64:607–613. - PMC - PubMed
    1. Bonavida B., Katz J., Gottlieb M. Mechanism of defective NK cell activity in patients with acquired immunodeficiency syndrome (AIDS) and AIDS-related complex. I. Defective trigger on NK cells for NKCF production by target cells and partial restoration by IL-2. J. Immunology. 1986;137:1157–1163. - PubMed

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