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. 2009 Oct 14;164(2-4):344-6.
doi: 10.1016/j.vetpar.2009.05.012. Epub 2009 May 19.

Improved method for Duddingtonia flagrans chlamydospores production for livestock use

Janio M Santurio  1 , Régis A Zanette, Aleksandro S Da Silva, Mario L De La Rue, Silvia G Monteiro, Sydney H Alves
Affiliations

Improved method for Duddingtonia flagrans chlamydospores production for livestock use

Janio M Santurio et al. Vet Parasitol. .

Abstract

Duddingtonia flagrans is a nematode-trapping fungus responsible for attacking larval stages of helminthes in pasture. D. flagrans chlamydospores were produced using a two-step liquid/solid culture system. The inoculum grown in liquid medium was transferred to a rice substrate and kept at room temperature for 30 days. Grains were washed, filtered and centrifuged. The pellet was lyophilized and the obtained yield averaged 1 x 10(5) chlamydospores per gram of dried material. The lyophilized chlamydospores showed a trapping rate of 69% of infective larvae in vitro and were excreted entirely in ovine faeces. The results showed that most of the chlamydospores remained intact and viable after the lyophilization process.

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