doi: 10.1364/opex.13.008855.
The efficiency of surface-plasmon coupled emission for sensitive fluorescence detection
- PMID: 19498918
- DOI: 10.1364/opex.13.008855
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The efficiency of surface-plasmon coupled emission for sensitive fluorescence detection
Opt Express.
.
Free article
Abstract
Surface-plasmon coupled emission (SPCE) has emerged as a new and potentially powerful tool for highly sensitive fluorescence detection. In the case of SPCE, the fluorescence is collected through a semi-transparent thin metal film deposited on glass. We present a theoretical analysis of SPCE, studying the potential enhancement of the fluorescence collection efficiency, brightness, quantum-yield, and photostability. The results are compared with fluorescence detection on a pure glass surface. It is shown that SPCE does not lead to any improvement, but that the metal film actually reduces the sensitivity of fluorescence detection.
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