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Comparative Study
. 2010 Feb;88(1):166-8.
doi: 10.1016/j.rvsc.2009.05.009. Epub 2009 Jun 5.

Comparison of enzyme-linked immunosorbent assay and RT-PCR for the detection of porcine epidemic diarrhoea virus

Enrica Sozzi  1 , Andrea Luppi, Davide Lelli, Ana Moreno Martin, Elena Canelli, Emiliana Brocchi, Antonio Lavazza, Paolo Cordioli
Affiliations
Comparative Study

Comparison of enzyme-linked immunosorbent assay and RT-PCR for the detection of porcine epidemic diarrhoea virus

Enrica Sozzi et al. Res Vet Sci. 2010 Feb.

Abstract

Porcine epidemic diarrhoea (PED) is a contagious enteric disease of pigs caused by a coronavirus. A double antibody sandwich enzyme-linked immunosorbent assay (DAS-ELISA) based on the use of monoclonal antibodies was developed for the detection of porcine epidemic diarrhoea virus (PEDV). The DAS-ELISA was compared with RT-PCR in the examination of 506 specimens collected during 2006-2007 from pigs originating from different farms located in the Po valley. Both faecal samples obtained directly from the rectum of live animals showing clinical signs and intestinal samples collected from the caecum of deceased pigs were included in the study. The correlation between the two methods was higher when testing faecal samples (K=0.97, 95% CI: 0.94-1.00) than testing intestinal samples (K=0.62, 95% CI: 0.35-0.89). The use of ELISA technology provided an efficient and effective mean of evaluating the presence of coronavirus PED antigen in field samples and indicates that this procedure is a very useful tool in epidemiological studies.

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Figures

Fig. 1
Fig. 1
Dose relationship between absorbance and sample dilutions. Absorbances for a weakly, mildly and strongly PEDV positive faecal samples and a negative faecal sample are shown.
See this image and copyright information in PMC

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