Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
Comment
. 2009 Jul 15;8(14):2300-2.
doi: 10.4161/cc.8.14.8852. Epub 2009 Jul 27.

Acetic acid effects on aging in budding yeast: are they relevant to aging in higher eukaryotes?

William C Burhans, Martin Weinberger
Comment

Acetic acid effects on aging in budding yeast: are they relevant to aging in higher eukaryotes?

William C Burhans et al. Cell Cycle. .
No abstract available

PubMed Disclaimer

Figures

Figure 1
Figure 1
Effects of buffering pH in yeast chronological aging experiments. (A) Viability of BY4741 wild type cells cultured in synthetic complete (SC) medium for indicated times in the absence (“unbuffered”) or presence (“buffered”) of 50 mM citrate-phosphate buffer (pH 6.0). The osmolarity of unbuffered medium was adjusted with sorbitol to maintain the same osmolarity as buffered medium. Viability was measured as colony-forming units. Data represent averages and standard deviations of three biological replicas. Unbuffered cultures achieved a final average pH of 3.19, and buffered cultures achieved a final average pH of 5.26. Similar results were obtained in a second genetic background (DBY746; not shown). (B) Reactive oxygen species in aliquots of 25,000 cells from buffered or unbuffered cultures were measured by flow cytometry at the indicated times of medium depletion using dihydroethidium (DHE). Y axis indicates number of cells producing DHE fluorescence signals in individual channels of the flow cytometer; X axis indicates channels detecting progressively higher levels of DHE fluorescence and is scaled exponentially. (C) The fraction of visibly budded cells (“% visible buds”) was determined microscopically at indicated times in aliquots of 500 or more cells from each time point. Data represent averages and standard deviations in three biological replicas. (D) Viability of cells with or without visible buds was assessed by staining aliquots of cells collected at indicated times with propidium iodide (PI) followed by microscopic examination with appropriate filters. Total cells counted in each sample exceeded 2,000 cells. Data are representative of results in 4 biological replicas. Additional PI staining data are presented in Figure S1.
See this image and copyright information in PMC

Comment on

References

    1. Burtner CR, et al. Cell Cycle. 2009;8:1256–70. - PMC - PubMed
    1. Allen C, et al. J Cell Biol. 2006;174:89–100. - PMC - PubMed
    1. Fabrizio P, et al. Genetics. 2003;163:35–46. - PMC - PubMed
    1. Weinberger M, et al. PLOS One. 2007;2:748.
    1. Mollapour M, et al. FEMS Yeast Res. 2006;6:1274–80. - PubMed

LinkOut - more resources