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Comment
. 2009 Jun 23;106(25):E64; author reply E65.
doi: 10.1073/pnas.0901936106. Epub 2009 Jun 8.

Nonsense suppression activity of PTC124 (ataluren)

Stuart W Peltz, Ellen M Welch, Allan Jacobson, Christopher R Trotta, Nikolai Naryshkin, H Lee Sweeney, David M Bedwell
Comment

Nonsense suppression activity of PTC124 (ataluren)

Stuart W Peltz et al. Proc Natl Acad Sci U S A. .
No abstract available

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Conflict of interest statement

Conflict of interest statement: S.W.P., E.M.W., A.J., C.R.T., N.N., L.L.S., and D.M.B. are employees or consultants of PTC Therapeutics, Inc.

Figures

Fig. 1.
Fig. 1.
PTC124 luciferase inhibition varies depending on the luciferase substrate used in the assay. (A) Inhibition of luciferase enzymatic activity is substrate-dependent. Recombinant luciferase protein (Sigma) was incubated with the indicated concentrations of PTC124 (synthesized by PTC Therapeutics). Luminescence was determined by using either the Bright-Glo or Steady-Glo luciferase substrate (Promega) as described by the manufacturer, and monitored with a Viewlux CCD imager (Perkin-Elmer). Luminescence data were normalized to reactions containing solvent alone (DMSO) and the percentage luciferase inhibition was calculated. Dotted line: 50% inhibition. (B) Inhibition of luciferase activity in the cell-based nonsense suppression assay is substrate-dependent. 293H cells were transiently transfected with a LUC nonsense suppression reporter gene containing a premature UGA terminator at codon 190 (2) and treated with the indicated concentrations of PTC124 for 20 h. After incubation, luminescence was determined by using either the Bright-Glo or Steady-Glo luciferase substrate as described for A. Luciferase activities were normalized to that produced with solvent alone (DMSO), and the fold suppression over background was calculated as PTC124light units/DMSOlight units.
See this image and copyright information in PMC

Comment on

References

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