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. 1977 Jul;59(1):273-5.
doi: 10.1093/jnci/59.1.273.

Isolation of T-lymphocytes from disaggregated tumors, with high purity and good percentage recovery

C B Hansen, G Y Gillespie, S W Russell

Isolation of T-lymphocytes from disaggregated tumors, with high purity and good percentage recovery

C B Hansen et al. J Natl Cancer Inst. 1977 Jul.

Abstract

A combination of two cell separation methods was utilized for the isolation of thymus-derived lymphocytes (TL) from enzymatically disaggregated tumors. Passage through Sephadex G-10-glass bead columns to remove adherent cell types followed by exposure to IgG-coated sheep red blood cell monolayers for removal of Fc receptor-bearing inflammatory cells provided functional TL suspensions of high purity with good percentage recovery.

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