Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2009 Oct;91(1):419-24.
doi: 10.1002/jbm.b.31417.

The use of collagen cross-linking agents to enhance dentin bond strength

Aiman Al-Ammar  1 , James L Drummond, Ana Karina Bedran-Russo
Affiliations

The use of collagen cross-linking agents to enhance dentin bond strength

Aiman Al-Ammar et al. J Biomed Mater Res B Appl Biomater. 2009 Oct.

Abstract

Type I collagen is a major component of the hybrid layer, and improvement of its mechanical properties may be advantageous during bonding procedures.

Objective: To investigate the effect of three different cross-linking agents (Glutaraldehyde [GD], Grape seed extract [GSE], and Genipin [GE]) on the tensile bond strength (TBS) of resin-dentin bonds.

Materials and methods: Sixty-four sound human molars were collected and their occlusal surfaces were ground flat to expose dentin. Dentin surfaces were etched using a phosphoric acid and then teeth were randomly divided according to the dentin treatment: Control group (no treatment), 5% GD, 6.5% GSE, or 0.5% GE. Teeth were restored either with One Step Plus or Adper Single Bond Plus adhesive systems and resin composite. After 24 h, teeth were sectioned to produce a cross-sectional surface area of 1.0 mm(2) and tested for tensile bond strength. Data were statistically analyzed using ANOVA and Fisher's PLSD tests (p < 0.05). There was a statistically significant interaction between factors (treatment and adhesive p < 0.001). Treatment affected TBS (p < 0.0001), while no differences were observed between the adhesive systems (p = 0.6961).

Conclusion: Chemical modification to the dentin matrix promoted by GD and GSE, but not GE, resulted in increased bond strength. The application of selective collagen cross-linkers during adhesive restorative procedures may be a new approach to improve dentin bond strength properties.

PubMed Disclaimer

Figures

Figure 1
Figure 1
Illustration of specimen selection (1), treatment (2), restorative procedures (3) and testing (4). GD (Glutaraldehyde); GSE (Grape seed extract); GE (Genipin).
Figure 2
Figure 2
Representative SEM images of the debonded interfaces treated with Control group, One Step Plus adhesive system. The most common fractured surface was at the bottom of the hybrid layer. White arrows: Hybrid layer; T: Resin tags.
Figure 3
Figure 3
Representative SEM images of the debonded interfaces treated with glutaraldehyde, One Step Plus system. Debonding pattern present at the adhesive layer/top hybrid layer. White arrows: Adhesive resin.
Figure 4
Figure 4
Representative SEM images of the debonded interfaces treated with grape seed extract, One Step Plus adhesive system. Debonding pattern present at the adhesive layer/top hybrid layer. White arrows: hybrid layer; black arrows: Dentin tubules.
Figure 5
Figure 5
Representative SEM images of the debonded interfaces treated with Genipin, One Step Plus adhesive system. Samples fractured at the bottom of hybrid layer. White arrows: peritubular dentin; Black arrows: Hybrid layer.
See this image and copyright information in PMC

Comment in

References

    1. Nakabayashi N, Saimi Y. Bonding to intact dentin. J Dent Res. 1996;75:1706–1716. - PubMed
    1. Nakabayashi N, Pashley DH. Hybridization of dental hard tissues. Tokyo, Japan: Quintessence; 1998. pp. 64–68.
    1. Van Noort R, Norooze S, Howard I, Cardew G. A critique of bond strength measurements. J Dent. 1989;17:61–67. - PubMed
    1. Van Noort R, Cardew G, Howard I, Norooze S. The effect of local interfacial geometry on the measurements of the tensile bond strength to dentin. J Dent Res. 1991;70:889–893. - PubMed
    1. Van Meerbeek B, De Munck J, Yosida Y, Inoue S, Vargas M, Vijay P, et al. Adhesion to enamel and dentin: current status and future challenges. Oper Dent. 2003;28:215–235. - PubMed

Publication types

LinkOut - more resources